PDE4A11 Antibody from MyBioSource.com

The PDE4 family of cAMP-specific phosphodiesterases represents an important therapeutic and pharmacological target for a variety of therpapeutic interventions including their anti-inflammatory and anti-depressant effects. Four distinct genes (PDE4A, PDE4B, PDE4C and PDE4D), encode large family of PDE4 isoforms [1, 2). Almost all of these isoforms have a common core made up of central catalytic domain and C-terminal tail. The differential RNA splicing and use of alternate initiation codons also generates the elongated N-terminal ends "longforms" PDE4 proteins. The long form PDE variants are characterized by upstream conserved regions (UCR1 and UCR2). The PDE4A10 is a rat/mouse homologue of long-form PDE4A variants. The expression of long-form PDE4A10 and PDE4A5 in CNS was mapped to hippocampus, striatum, and thalamus. The long-forms are consipicously absent in the cerebellum and brain stem (3). The expression of shortform PDE4A1 was evident in glomerular layer of the olfacotry bulb, CA4 pyramidal region and cerebellum. PDE4A isoforms are present in several key areas of the brain and therefore present valid targets for therapeutic interventions. PDE4A10-selective antibody was generated against unique N-terminal sequence, the peptide was post-synthetically modified to achieve maximum antigeneicty and provide proper linkers for covalent coupling to carrier protien (blue carrier protien). Antibody PD4-161AP is also available in affinity-purified form and as HRP conjugate (PD4-162HRP; inquire) for western blotting and immunnohistochemical localization experiments. MyBioSource provides PDE family selective, family subtype-selective and subtype-variant selective antibodies for detailed analyses of cAMP signaling pathways. The peptide was conjugated with BCP for the production of antibodies in rabbits. Antibody PD4-170P and PD4-171AP labels PDE4A11 protein in PC-PD4A11 samples and in rat hippocampal extracts. The PDE4A11 variant specific antibody does not cross react with PDE4A10, PDE4A8 or other PDE4A proteins including PDE4A4, PDE4A1 and PDE4Ax or with other PDE family members. MyBioSource carries Western blot positive controls for PDE4A11 in ready-to-use buffers for easy identification and quantification of PDE4A11 protein in samples. MyBioSource also offers services to tag a fluorophore or make custom affinity matrices using these antibodies